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customarraytm 4x2k mirna array  (CombiMatrix)
90
CombiMatrix customarraytm 4x2k mirna array
A ) Schematic representation of the experimental design. More than 100 five-week-old tobacco plants were inoculated with TMV or with mock solution in the fifth leaf. Third upper leaves (leaves number 8) were sampled at 1, 5, 8, 15 and 22 days post inoculation (dpi). Each sample was ground and the powder divided in 4 parts for virus detection by ELISA, <t>miRNA</t> detection by microarray and for primary and secondary metabolites detection by GC-MS and LC-MS respectively. B ) TMV infection progression along the experiment in virus-inoculated plants. Bar graph: percentage of TMV-inoculated plants systemically accumulating virus as detected by ELISA in the sampled leaf along the infection. Squares line: Relative systemic accumulation of viral CP quantified by ELISA assays for samples at each time point. Diamonds line: percentage of TMV-inoculated plants showing characteristic symptoms under naked eye inspection. n = 10 plants per each time point.
Customarraytm 4x2k Mirna Array, supplied by CombiMatrix, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/customarraytm 4x2k mirna array/product/CombiMatrix
Average 90 stars, based on 1 article reviews
customarraytm 4x2k mirna array - by Bioz Stars, 2026-05
90/100 stars
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oligonucleotide probes  (CombiMatrix)
90
CombiMatrix oligonucleotide probes
A ) Schematic representation of the experimental design. More than 100 five-week-old tobacco plants were inoculated with TMV or with mock solution in the fifth leaf. Third upper leaves (leaves number 8) were sampled at 1, 5, 8, 15 and 22 days post inoculation (dpi). Each sample was ground and the powder divided in 4 parts for virus detection by ELISA, <t>miRNA</t> detection by microarray and for primary and secondary metabolites detection by GC-MS and LC-MS respectively. B ) TMV infection progression along the experiment in virus-inoculated plants. Bar graph: percentage of TMV-inoculated plants systemically accumulating virus as detected by ELISA in the sampled leaf along the infection. Squares line: Relative systemic accumulation of viral CP quantified by ELISA assays for samples at each time point. Diamonds line: percentage of TMV-inoculated plants showing characteristic symptoms under naked eye inspection. n = 10 plants per each time point.
Oligonucleotide Probes, supplied by CombiMatrix, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/oligonucleotide probes/product/CombiMatrix
Average 90 stars, based on 1 article reviews
oligonucleotide probes - by Bioz Stars, 2026-05
90/100 stars
  Buy from Supplier

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A ) Schematic representation of the experimental design. More than 100 five-week-old tobacco plants were inoculated with TMV or with mock solution in the fifth leaf. Third upper leaves (leaves number 8) were sampled at 1, 5, 8, 15 and 22 days post inoculation (dpi). Each sample was ground and the powder divided in 4 parts for virus detection by ELISA, miRNA detection by microarray and for primary and secondary metabolites detection by GC-MS and LC-MS respectively. B ) TMV infection progression along the experiment in virus-inoculated plants. Bar graph: percentage of TMV-inoculated plants systemically accumulating virus as detected by ELISA in the sampled leaf along the infection. Squares line: Relative systemic accumulation of viral CP quantified by ELISA assays for samples at each time point. Diamonds line: percentage of TMV-inoculated plants showing characteristic symptoms under naked eye inspection. n = 10 plants per each time point.

Journal: PLoS ONE

Article Title: Metabolic and miRNA Profiling of TMV Infected Plants Reveals Biphasic Temporal Changes

doi: 10.1371/journal.pone.0028466

Figure Lengend Snippet: A ) Schematic representation of the experimental design. More than 100 five-week-old tobacco plants were inoculated with TMV or with mock solution in the fifth leaf. Third upper leaves (leaves number 8) were sampled at 1, 5, 8, 15 and 22 days post inoculation (dpi). Each sample was ground and the powder divided in 4 parts for virus detection by ELISA, miRNA detection by microarray and for primary and secondary metabolites detection by GC-MS and LC-MS respectively. B ) TMV infection progression along the experiment in virus-inoculated plants. Bar graph: percentage of TMV-inoculated plants systemically accumulating virus as detected by ELISA in the sampled leaf along the infection. Squares line: Relative systemic accumulation of viral CP quantified by ELISA assays for samples at each time point. Diamonds line: percentage of TMV-inoculated plants showing characteristic symptoms under naked eye inspection. n = 10 plants per each time point.

Article Snippet: Combimatrix CustomArrayTM 4X2K miRNA array was used, each slide has four identical array sectors that can be hybridized with different microRNA samples simultaneously, each array sector carry anti-sense oligonucleotide probes with a median length of 22 nucleotides and an average Tm of 55 to 60°C.

Techniques: Virus, Enzyme-linked Immunosorbent Assay, Microarray, Gas Chromatography-Mass Spectrometry, Liquid Chromatography with Mass Spectroscopy, Infection

Systemic, non-inoculated tobacco leaves were sampled from virus- and mock-inoculated plants (n = 6 for each day and treatment) at 5, 15 and 22 dpi. miRNAs accumulation in TMV-inoculated plants were compared to those corresponding to mock-inoculated plants and the relative accumulation was calculated as the ratio between virus- and mock-inoculated samples at each time point. These ratios were log2 transformed and further analyzed with hierarchical clustering with average linkage. False-color scale indicates lower (green) or greater (red) miRNA accumulation in TMV-infected plants respect to mock-inoculated ones. The horizontal bar shows the log2-based scale. See for further details.

Journal: PLoS ONE

Article Title: Metabolic and miRNA Profiling of TMV Infected Plants Reveals Biphasic Temporal Changes

doi: 10.1371/journal.pone.0028466

Figure Lengend Snippet: Systemic, non-inoculated tobacco leaves were sampled from virus- and mock-inoculated plants (n = 6 for each day and treatment) at 5, 15 and 22 dpi. miRNAs accumulation in TMV-inoculated plants were compared to those corresponding to mock-inoculated plants and the relative accumulation was calculated as the ratio between virus- and mock-inoculated samples at each time point. These ratios were log2 transformed and further analyzed with hierarchical clustering with average linkage. False-color scale indicates lower (green) or greater (red) miRNA accumulation in TMV-infected plants respect to mock-inoculated ones. The horizontal bar shows the log2-based scale. See for further details.

Article Snippet: Combimatrix CustomArrayTM 4X2K miRNA array was used, each slide has four identical array sectors that can be hybridized with different microRNA samples simultaneously, each array sector carry anti-sense oligonucleotide probes with a median length of 22 nucleotides and an average Tm of 55 to 60°C.

Techniques: Virus, Transformation Assay, Infection

Relative accumulation of miRNAs 156, 164, 165/6, 167 and 171 ( A,B ), and miRNAs 156, 166, and 171 target genes ( C,D ) on virus-infected plants with low inoculum . A ratio between each virus- and mock-inoculated plants is shown at 6 days post inoculation (dpi) ( A,C ) and 11 dpi ( B,D ). * means p-value<0.1; ** means p-value<0.05 and *** means p-value<0.01.

Journal: PLoS ONE

Article Title: Metabolic and miRNA Profiling of TMV Infected Plants Reveals Biphasic Temporal Changes

doi: 10.1371/journal.pone.0028466

Figure Lengend Snippet: Relative accumulation of miRNAs 156, 164, 165/6, 167 and 171 ( A,B ), and miRNAs 156, 166, and 171 target genes ( C,D ) on virus-infected plants with low inoculum . A ratio between each virus- and mock-inoculated plants is shown at 6 days post inoculation (dpi) ( A,C ) and 11 dpi ( B,D ). * means p-value<0.1; ** means p-value<0.05 and *** means p-value<0.01.

Article Snippet: Combimatrix CustomArrayTM 4X2K miRNA array was used, each slide has four identical array sectors that can be hybridized with different microRNA samples simultaneously, each array sector carry anti-sense oligonucleotide probes with a median length of 22 nucleotides and an average Tm of 55 to 60°C.

Techniques: Virus, Infection

A ) Predicted fold-back secondary structures of tobacco and tomato miRNA166a precursors as determined by the RNAfold program. Mature miRNA sequences are highlighted in green. Clustal alignment between Pre-miRNA166a sequences from tobacco and tomato. ( BC ) Pre-miRNA166a qPCR quantification in plants infected with TMV or ShMV, ( B ) 6 dpi and ( C ) 11 dpi. ** means p-value<0,05 and *** means p-value<0,01.

Journal: PLoS ONE

Article Title: Metabolic and miRNA Profiling of TMV Infected Plants Reveals Biphasic Temporal Changes

doi: 10.1371/journal.pone.0028466

Figure Lengend Snippet: A ) Predicted fold-back secondary structures of tobacco and tomato miRNA166a precursors as determined by the RNAfold program. Mature miRNA sequences are highlighted in green. Clustal alignment between Pre-miRNA166a sequences from tobacco and tomato. ( BC ) Pre-miRNA166a qPCR quantification in plants infected with TMV or ShMV, ( B ) 6 dpi and ( C ) 11 dpi. ** means p-value<0,05 and *** means p-value<0,01.

Article Snippet: Combimatrix CustomArrayTM 4X2K miRNA array was used, each slide has four identical array sectors that can be hybridized with different microRNA samples simultaneously, each array sector carry anti-sense oligonucleotide probes with a median length of 22 nucleotides and an average Tm of 55 to 60°C.

Techniques: Infection

Metabolites-metabolite (AB) or miRNA-metabolites level (CD) correlation was calculated between inoculated plants (AC) or mock-inoculated plants (BD). Significant correlation ( p = 0.001, for AB and p = 0.01 for CD; R>0.5) between two metabolites was drawn with color lines. Positive correlations were drawn with red lines, negative with blue lines. Correlations present in both TMV inoculated (AC) and mock-inoculated plants (BD) were drawn in black, regardless the sense of the correlation.

Journal: PLoS ONE

Article Title: Metabolic and miRNA Profiling of TMV Infected Plants Reveals Biphasic Temporal Changes

doi: 10.1371/journal.pone.0028466

Figure Lengend Snippet: Metabolites-metabolite (AB) or miRNA-metabolites level (CD) correlation was calculated between inoculated plants (AC) or mock-inoculated plants (BD). Significant correlation ( p = 0.001, for AB and p = 0.01 for CD; R>0.5) between two metabolites was drawn with color lines. Positive correlations were drawn with red lines, negative with blue lines. Correlations present in both TMV inoculated (AC) and mock-inoculated plants (BD) were drawn in black, regardless the sense of the correlation.

Article Snippet: Combimatrix CustomArrayTM 4X2K miRNA array was used, each slide has four identical array sectors that can be hybridized with different microRNA samples simultaneously, each array sector carry anti-sense oligonucleotide probes with a median length of 22 nucleotides and an average Tm of 55 to 60°C.

Techniques: